Association of SNP rs1447295 and microsatellite marker DG8S737 with familial prostate cancer and high grade disease.

The Journal of urology 2010 Aug 1; 184(2):738-42

Hamano T, Matsui H, Sekine Y, Ohtake N, Nakata S, Suzuki K

Department of Urology, Gunma University School of Medicine, Maebashi, Gunma, Japan.

PURPOSE: Several prior studies show a relationship between genetic markers at chromosome 8q24 and an increased prostate cancer risk. We confirmed the association of 8q24 markers with prostate cancer in the Japanese population and the association of these genetic variants with clinical characteristics. MATERIALS AND METHODS: Included in this study were 134 patients with familial prostate cancer, 158 with sporadic prostate cancer and 119 controls. All were Japanese. We genotyped the 2, 8q24 markers SNP rs1447295 and microsatellite marker DG8S737 using real-time polymerase chain reaction and polymerase chain reaction based assay with fluorescence labeled primers. RESULTS: There was a significant positive association between the DG8S737 -12 allele and familial prostate cancer risk (OR 1.86, 95% CI 1.11-3.00, p = 0.02) and a significant association of risk with the rs1447295 A allele (OR 2.36, 95% CI 1.41-3.94, p = 0.002). Significant associations were noted for each marker in men with a high Gleason score. CONCLUSIONS: Two alleles at 8q24 are genetic risk factors for familial prostate cancer and high grade disease.

Keywords: rs1447295 DG8S737 prostate cancer biomarker