Metformin inhibits inflammatory angiogenesis in a murine sponge model.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2009 Oct 24; In press
Xavier D DO, Amaral L LS, Gomes M MA, Rocha M MA, Campos P PR, Cota B BD, Tafuri L LS, Paiva A AM, Silva J JH, Andrade S SP, Belo A AV
FUMEC University, Physiology, Rua da Paisagem, 240, Bairro Vila da Serra, 34000-000 Nova Lima, Minas Gerais, Brazil.
To investigate the effects of metformin on angiogenesis, on inflammatory cell accumulation and on production of endogenous cytokines in sponge implant in mice. Polyester-polyurethane sponges were implanted in Swiss mice and metformin (40 or 400mg/kg/day) was given orally for six days. The implants collected at day 7 postimplantation were processed for the assessment of hemoglobin (Hb), myeloperoxidase (MPO), N-acetylglucosaminidase (NAG) e collagen used as indexes for angiogenesis, neutrophil and macrophage accumulation and extracellular matrix deposition, respectively. Relevant inflammatory, angiogenic and fibrogenic cytokines were also determined. Metformin treatment attenuated the main components of the fibrovascular tissue, wet weight, vascularization (Hb content), macrophage recruitment (NAG activity), collagen deposition and the levels of transforming growth factor (TGF-beta1) intraimplant. A regulatory function of metformin on multiple parameters of main components of inflammatory angiogenesis has been revealed giving insight into the potential therapeutic underlying the actions of metformin.
Keywords: Metformin angiogenesis