Anti-norovirus polyclonal antibody and its potential for development of an antigen-ELISA.
Journal of medical virology 2007 Aug 1; 79(8):1180-6
Okame M, Shiota T, Hansman G, Takagi M, Yagyu F, Takanashi S,
Department of Developmental Medical Sciences, Graduate School of Medicine, The University of Tokyo,
Norovirus (NoV) capsid proteins were expressed as virus-like particles (VLPs) by using recombinant baculovirus in insect cells, which had 5 genotypes in genogroup I and 11 genotypes in genogroup II, and the VLPs were used as immunogens. Polyclonal antibody against the VLP of GII/3 genotype showed broad-range cross-reactivity, reacting not only with intra-genogroup strains, but also inter-genogroup strains, by antibody-ELISA using 16 kinds of VLPs. Furthermore, antigen-ELISA was conducted in sandwich enzyme-linked immunosorbent assay (ELISA) using the polyclonal antibody for capturing antigens, and three kinds of monoclonal antibodies against the VLP of GII/4 genotype for detecting antigens. This format successfully detected eight genotypes of NoV from clinical specimens and proved that polyclonal antibody, which has broad-range cross-reactivity, was capable of detecting various types of genotypes from clinical specimens. J. Med. Virol. 79: 1180-1186, 2007. (c) 2007 Wiley-Liss, Inc.