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Aptamer selection for the inhibition of cell adhesion with fibronectin as target.

Bioorganic & medicinal chemistry letters 2004 Aug 2; 14(15):4001-4

Link to PubMed abstract

Ogawa A A, Tomita N N, Kikuchi N N, Sando S S, Aoyama Y Y

Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-Ku, Kyoto 615-8510, Japan.

An affinity column immobilizing a decapeptide H(2)N-RGDSPASSKP-CO(2)H was used to select RGD-binding aptamers from a pool of 86-mer single-strand oligodeoxynucleotides (ODNs) containing a random 40-mer sequence. The enriched library thus obtained was further selected against adsorbed fibronectin and individual aptamers were monocloned in E. coli and sequenced to give a couple of highly homologous ODNs, which indeed inhibited fibronectin-integrin mediated cell adhesion.